Optimization and Validation of a QuEChERS-Based Method Combined with Gas Chromatography-Tandem Mass Spectrometry for...

The increasing popularity of edible insects as a sustainable food source necessitates stringent safety measures to monitor pesticide contamination. This study aimed to assess and enhance a QuEChERS-based extraction method coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) for the quantification of pesticide residues in edible insects (bamboo caterpillars, house crickets, silkworm pupae, giant water bugs, and grasshoppers) by combining multiple individual insect specimens into a single, homogenized sample-five replicates were tested. The method was optimized by evaluating various extraction parameters and showed strong linearity for all 47 target pesticides, with correlation coefficients (R²) ranging from 0.9940 to 0.9999. The limits of detection (LODs) varied between 1 and 10 µg/kg, while the limits of quantification (LOQs) ranged from 10 to 15 µg/kg. Recovery studies conducted at three fortification levels (10, 100, and 500 µg/kg) revealed recoveries ranging from 64.54% to 122.12%, that over 97.87% of the pesticides exhibited satisfactory recoveries within the range of 70-120%, and relative standard deviations (RSDs) below 20%, between 1.86% and 6.02%. Matrix effects (%MEs) range from -33.01% to 24.04%, and to those that experienced no effect. More than 94% of the analytes showed minimal ion suppression or enhancement. These results conform to the SANTE guidelines for monitoring pesticide residues in edible insects, enhancing food safety standards and safeguarding consumer protection.