Related Experiment Video
Updated: Jun 16, 2025
Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Published on: September 17, 2017
Proteomic Analysis of Listeria monocytogenes Subjected to Pulsed Magnetic Field
Di Chen1, Jingya Qian1, Shuhao Huo1
1School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, China.
Abstract:
As one of the non-thermal technologies, the pulsed magnetic field (PMF) has increasingly attracted attention for its application in food microbial inactivation. In this study, a proteomic analysis was conducted to elucidate the molecular mechanism underlying the inactivation of Listeria monocytogenes (L. monocytogenes) by a PMF. A total of 79 proteins, comprising 65 upregulated and 14 downregulated proteins, were successfully identified as differentially expressed proteins (DEPs, >1.2-fold or <0.83-fold, p-value < 0.05) in Listeria monocytogenes exposed to a PMF at 8 T with 20 pulses. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that PMF exposure significantly impacted nutrient transport, the composition of cytoplasmic and intracellular substances, and various metabolic processes in L. monocytogenes, such as carbohydrate metabolism, amino acid metabolism, and nicotinate and nicotinamide metabolism. The disruption of cellular functions and metabolic pathways may contribute to the death of L. monocytogenes under PMF treatment. These findings provide valuable insights and serve as a reference for further investigations into the inactivation mechanisms induced by PMFs.
Insights
Pulsed magnetic field (PMF) treatment inactivates Listeria monocytogenes by disrupting key cellular functions. Proteomic analysis revealed significant changes in nutrient transport and metabolism, offering insights into microbial inactivation mechanisms.
Area of Science:
- Microbiology and Food Science
- Proteomics and Molecular Biology
Background:
- Pulsed magnetic fields (PMF) are a non-thermal technology gaining interest for food microbial inactivation.
- Understanding the molecular mechanisms of PMF's effect on bacteria like Listeria monocytogenes is crucial for its application.
Purpose of the Study:
- To elucidate the molecular mechanisms behind Listeria monocytogenes inactivation using pulsed magnetic field (PMF) treatment.
- To identify differentially expressed proteins (DEPs) in L. monocytogenes upon exposure to PMF.
Main Methods:
- Proteomic analysis was performed on Listeria monocytogenes exposed to a 8 T, 20-pulse PMF.
- Differentially expressed proteins (DEPs) were identified using a fold change threshold of >1.2 or <0.83 and a p-value < 0.05.
- Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted.
Main Results:
- A total of 79 differentially expressed proteins (DEPs) were identified in L. monocytogenes after PMF exposure.
- PMF treatment significantly upregulated 65 proteins and downregulated 14 proteins.
- Affected cellular processes included nutrient transport, cytoplasmic/intracellular substance composition, and metabolic pathways (carbohydrate, amino acid, nicotinate/nicotinamide metabolism).
Conclusions:
- PMF exposure disrupts critical cellular functions and metabolic pathways in Listeria monocytogenes.
- These disruptions likely contribute to the observed microbial inactivation.
- Findings provide a foundation for further research into PMF-based microbial control strategies.
