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Updated: May 30, 2026

Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut

Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut

Published on: February 14, 2011

Virus Isolation from Field-Collected Ticks: In Vivo.

Jessica Crooker^1,2,3, Dakota N Paine^1,2,3, Saravanan Thangamani^4,5

¹Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 13, 2025

Summary

This summary is machine-generated.

Powassan virus (POWV) and Deer tick virus (DTV) are emerging North American tick-borne viruses. This study presents a new protocol for isolating these viruses from field-collected ticks using an in vivo system.

Keywords:

Deer tick virus Powassan virus Tick-borne disease Tick-borne virus Viral isolation

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Published on: April 6, 2022

Area of Science:

Virology
Epidemiology
Public Health

Background:

Tick-borne viruses like Powassan virus (POWV) and Deer tick virus (DTV) are increasing in prevalence.
These viruses are genetically similar but distinct emerging threats in North America, causing severe encephalitis.
Current research often relies on lab strains, potentially not reflecting circulating viral populations.

Purpose of the Study:

To develop and present a reliable protocol for isolating circulating strains of POWV and DTV from field-collected ticks.
To facilitate better understanding of viral evolution and more accurate human health risk assessments.
To enhance the translational potential of research on these emerging tick-borne viruses.

Main Methods:

The study outlines a protocol for isolating POWV and DTV.
The methodology utilizes an in vivo system for virus isolation.
Field-collected ticks are the source material for isolating circulating viral strains.

Main Results:

The protocol enables reliable isolation of POWV and DTV from field-collected ticks.
This method allows for the study of circulating viral strains, not just laboratory prototypes.
The findings support improved understanding of viral diversity and epidemiology.

Conclusions:

The presented in vivo protocol offers a reliable method for isolating emerging tick-borne viruses like POWV and DTV.
This approach is crucial for studying viral evolution and assessing public health risks associated with these pathogens.
The methodology advances translational research for tick-borne encephalitis viruses.