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Updated: Jun 15, 2025
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Published on: March 3, 2016
Protocol for differentiation of human pluripotent stem cells into definitive endoderm lineage in a chemically defined
He En1, Yijie Guo1, Zhiju Zhao2
1Zhongshan School of Medicine and the Seventh Affiliated Hospital, Sun Yat-Sen University, Guangdong 510080, China; Key Laboratory for Stem Cells and Tissue Engineering (Sun Yat-Sen University), Ministry of Education, Guangdong 510080, China.
Abstract:
Here, we present a protocol for the differentiation of human pluripotent stem cells (hPSCs) into definitive endoderm (DE) lineage. We describe steps for the resuscitation, passaging, and plating of hPSCs. We then detail procedures for culturing cells followed by immunostaining, imaging, and analysis. This chemically defined, small-molecule-based, recombinant protein-free system offers a cost-effective and scalable platform for generating endodermal derivatives, demonstrating efficiency for applications in drug screening, disease modeling, and regenerative medicine. For complete details on the use and execution of this protocol, please refer to Zhao et al.1.
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