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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
Published on: December 9, 2013
Fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy
Abstract:
We report the application of fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy. This novel, to our knowledge, label-free imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth-harmonic and five-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.
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