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  1. Home
  3. Anti-biofilm Activity Of Lysostaphin-functionalized Titanium Surfaces Against Staphylococcus Aureus.
  1. Home
  3. Anti-biofilm Activity Of Lysostaphin-functionalized Titanium Surfaces Against Staphylococcus Aureus.

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Anti-biofilm activity of lysostaphin-functionalized titanium surfaces against Staphylococcus aureus.

Arathi T P1, Jayalakshmi Jayakumar1, Vivek Vinod1

  • 1School of Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi, 682041, Kerala, India.

Microbial Pathogenesis
|June 13, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Covalently attaching lysostaphin (Lst) to titanium (Ti) surfaces creates an effective barrier against Staphylococcus aureus biofilms. This Lst-Ti surface prevents infections on medical implants without releasing antimicrobial agents.

Keywords:
BiofilmCatheterConjugationLysostaphinProteinStaphylococcus aureus

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Area of Science:

  • Biomaterials Science
  • Infectious Diseases
  • Microbiology

Background:

  • Staphylococcus aureus forms antibiotic-resistant biofilms on medical implants.
  • Implant-associated infections pose significant risks to patients.

Purpose of the Study:

  • To investigate the covalent attachment of lysostaphin (Lst) to titanium (Ti) surfaces.
  • To evaluate the antibiofilm efficacy and biocompatibility of Lst-Ti surfaces.

Main Methods:

  • Covalent attachment of lysostaphin to titanium surfaces.
  • Characterization using SEM-EDS.
  • Biocompatibility assessment via cell viability and hemolytic activity tests.
  • Antimicrobial assays against Staphylococcus aureus and MRSA biofilms.
  • Biofilm reduction analysis using Scanning Electron Microscopy.

Main Results:

  • Successful covalent attachment of lysostaphin to Ti surfaces confirmed by SEM-EDS.
  • Lst-Ti surfaces demonstrated excellent cytocompatibility and minimal hemolytic activity.
  • Significant inhibition of S. aureus growth and disruption of established biofilms, including MRSA.
  • Reduced biofilm formation on Lst-Ti surfaces validated by SEM.

Conclusions:

  • Lst-Ti surfaces present a promising strategy for preventing and treating implant-associated biofilm infections.
  • This approach offers long-term antimicrobial protection without protein release, mitigating antibiotic resistance and systemic absorption risks.
  • Potential to improve patient outcomes by reducing implant removal necessity.