Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fast Reactions01:27

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-Performance SiPM Detection Module for Ultra-Fast Time-Resolved Measurements.

Sensors (Basel, Switzerland)·2026
Same author

Up to 200% of the excitation frequency in compressive FLIM: a constraint-free pile-up correction methodology for advanced biomedical imaging.

Optics express·2025
Same author

Pile-up free fluorescence lifetime imaging with a SPAD-based single pixel camera.

Optics express·2025
Same author

Laser-written reconfigurable photonic integrated circuit directly coupled to a single-photon avalanche diode array.

Light, science & applications·2025
Same author

Near-zero distortion in TCSPC at more than one photon per excitation period: experimental validation.

Optics letters·2024
Same author

Integrated Active Quenching Circuit for High-Rate and Distortionless SPAD-Based Time-Resolved Fluorescence Applications.

IEEE transactions on biomedical circuits and systems·2024

Related Experiment Video

Updated: Jul 17, 2026

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

8.8K

Speed-optimization strategies for time-correlated single photon counting experiments.

Alessandro Cominelli, Giulia Acconcia, Ivan Rech

    Optics Express
    |June 14, 2025
    PubMed
    Summary

    Time-correlated single photon counting (TCSPC) can be significantly accelerated by overcoming pile-up and dead-time limitations. This study explores advanced techniques and provides a methodology to maximize TCSPC measurement speed for faster scientific discovery.

    More Related Videos

    A Protocol for Real-time 3D Single Particle Tracking
    10:16

    A Protocol for Real-time 3D Single Particle Tracking

    Published on: January 3, 2018

    14.9K
    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.3K

    Related Experiment Videos

    Last Updated: Jul 17, 2026

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
    06:08

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

    Published on: December 27, 2018

    8.8K
    A Protocol for Real-time 3D Single Particle Tracking
    10:16

    A Protocol for Real-time 3D Single Particle Tracking

    Published on: January 3, 2018

    14.9K
    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
    08:43

    A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

    Published on: December 1, 2018

    11.3K

    Area of Science:

    • Physics
    • Photonics
    • Instrumentation

    Background:

    • Time-correlated single photon counting (TCSPC) is a crucial technique for analyzing fast luminous signals with picosecond accuracy.
    • Existing TCSPC setups face speed limitations due to pile-up and dead-time effects, restricting photon detection rates.

    Purpose of the Study:

    • To provide a comprehensive understanding of the maximum achievable speed in TCSPC experiments.
    • To analyze advanced techniques and technologies for enhancing TCSPC measurement speed.
    • To propose a methodology for calculating optimal speed and selecting appropriate technologies.

    Main Methods:

    • Review of state-of-the-art fast detectors and electronics with reduced dead times and multi-hit capabilities.
    • Analysis of advanced techniques designed to mitigate distortion phenomena in TCSPC.
    • Development of a step-by-step methodology for speed calculation and technology selection.

    Main Results:

    • Identified key limitations in TCSPC speed, primarily pile-up and dead-time phenomena.
    • Highlighted the impact of fast detectors, low dead-time electronics, and multi-hit capabilities on speed.
    • Demonstrated that advanced techniques can overcome distortions and enable higher photon rates.

    Conclusions:

    • Significant advancements in TCSPC technology allow for unprecedented measurement speeds.
    • The proposed methodology aids researchers in optimizing TCSPC experiments for maximum throughput.
    • Pushing TCSPC speed is critical for applications in life sciences and remote sensing.