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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...

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Related Experiment Video

Updated: May 11, 2026

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
13:56

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

Published on: July 18, 2013

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Activity-Based Protein Profiling to Study Virus-Host and Viral-Induced MicroRNA-Host Interactions.

Parrish Evers1, John Paul Pezacki2

  • 1Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|June 14, 2025
PubMed
Summary
This summary is machine-generated.

Activity-based protein profiling (ABPP) helps study virus-host interactions and microRNA roles in viral propagation. This method identifies new therapeutic targets and antiviral inhibitors for infectious diseases.

Keywords:
Activity-based protein profiling (ABPP)Chemical ProteomicsChemoproteomicsHepatic lipase (LIPC)In-gel fluorescenceMass spectrometryVirusWestern blottingmicroRNA

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Area of Science:

  • Biochemistry
  • Virology
  • Molecular Biology

Background:

  • Activity-based protein profiling (ABPP) is a powerful technique for investigating protein function in disease.
  • Viruses exploit host cell machinery, including noncoding RNAs like microRNAs, for replication.
  • Understanding virus-host interactions is crucial for developing antiviral strategies.

Purpose of the Study:

  • To describe a protocol for using ABPP to study viral infections in cells.
  • To outline methods for analyzing ABPP data, including in-gel fluorescence, western blotting, and mass spectrometry.
  • To highlight the potential of ABPP in identifying novel therapeutic targets and antiviral inhibitors.

Main Methods:

  • Cell infection and transfection protocols for ABPP.
  • Application of ABPP using in-gel fluorescence for protein activity visualization.
  • Western blotting and mass spectrometry for detailed analysis of protein targets.

Main Results:

  • Demonstration of ABPP's utility in studying virus-host interactions.
  • Identification of key protein targets affected by viral infection.
  • Validation of ABPP for assessing the impact of microRNAs in viral propagation.

Conclusions:

  • ABPP provides a robust platform for dissecting the functional consequences of viral infections at the proteome level.
  • The described methods enable the discovery of new therapeutic targets for antiviral drug development.
  • ABPP facilitates the evaluation of novel antiviral compounds by monitoring target engagement and efficacy.