Related Experiment Video
Updated: Jun 16, 2025
07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
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Protocol for sub-mucosal orthotopic injection of organoids into murine colon to study tumor growth and metastasis
Sura Atatri1, Marianne Meyers1, Eliane Klein1
1Department of Life Sciences and Medicine, University of Luxembourg, L-4367 Belvaux, Luxembourg.
STAR Protocols
|June 14, 2025
Summary
This study presents a colonoscopy-guided method for transplanting colorectal cancer (CRC) organoids into mice. This technique enhances the study of CRC tumor development and treatment responses in a more physiologically relevant model.
Area of Science:
- Oncology
- Gastroenterology
- Translational Medicine
Background:
- Orthotopic mouse models are crucial for studying colorectal cancer (CRC) progression.
- Existing models may not fully replicate the complex tumor microenvironment and metastatic processes observed in human patients.
Purpose of the Study:
- To provide a detailed protocol for colonoscopy-guided organoid transplantation into the murine colon.
- To establish a more physiologically relevant orthotopic model for colorectal cancer research.
Main Methods:
- Development of a protocol for preparing mouse organoids, equipment, and recipient mice.
- Execution of colonoscopy-guided mucosal injections into the murine colon.
- Description of post-injection care and monitoring.
Main Results:
- Successful establishment of a reproducible colonoscopy-guided organoid transplantation technique.
- Demonstration of the model's utility for studying CRC survival, metastasis, and therapeutic interventions.
Conclusions:
- Colonoscopy-guided organoid transplantation offers a superior method for creating orthotopic colorectal cancer models in mice.
- This refined model facilitates in-depth investigation of CRC pathogenesis and treatment efficacy.
