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Updated: Aug 5, 2026
Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets
Published on: July 18, 2019
Protocol to stress human primary pancreatic islets with diabetogenic factors for subsequent single-cell RNA
Daniel A Veronese-Paniagua1, Marlie M Maestas1, Jared P Taylor2
1Roy and Diana Vagelos Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA; Division of Endocrinology, Metabolism, and Lipid Research, Washington University School of Medicine, St. Louis, MO 63110, USA.
Abstract:
Type 1 diabetes (T1D) is an autoimmune disease that destroys insulin-secreting pancreatic β cells. Using human islets is instrumental to understanding the initiation and progression of T1D. Here, we present a protocol for treating cadaveric primary human islets with inflammatory, immune, and viral stressors. We detail Coxsackievirus B3 expansion, primary islet cell culture, and treatment with diabetogenic stressors, followed by procedures for single-cell RNA sequencing analysis. For complete execution details, please refer to Maestas et al.1 and Veronese-Paniagua et al.2.
