Lipid droplet-targeted AIEgen with dual-responsiveness for sulfur dioxide and viscosity monitoring in live-cell imaging and food analysis

  • 1The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, China.
  • 2Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.
  • 3Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China; Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, South China University of Technology, Guangzhou 510640, China. Electronic address: pshzhang07@gmail.com.
  • 4The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, China. Electronic address: panying@gdmu.edu.cn.
  • 5The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523808, China. Electronic address: jianqiangliu2010@126.com.

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Abstract

Sulfur dioxide (SO2) and viscosity play pivotal roles in regulating the physiological function within organisms. The abnormal levels of SO2 and viscosity can lead to various diseases, emphasizing the need for reliable detection methods in biological and real food samples. Herein, we introduce a lipid droplet-targeted novel triphenylamine-based fluorescent probe, TPA-CN, which exhibits aggregation-induced emission (AIE) properties for dual response to SO2 and viscosity. The TPA-CN could ratiometrically recognize SO2 with several advantages, such as excellent selectivity and anti-interference, rapid response rate, good sensitivity, and wide pH adaptability. The TPA-CN was also applied to monitor the changes in viscosity through the twisted intramolecular charge transfer (TICT) mechanism. In addition, bioimaging results demonstrated that TPA-CN can effectively monitor endogenous and exogenous SO₂ as well as viscosity in real time, with specific targeting of lipid droplets. Moreover, TPA-CN facilitates determining SO2 levels in water and food samples, demonstrating robust recovery performance.

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