Related Experiment Video
Updated: May 12, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Xylose reductase generated by yeast Candida tropicalis purified using an aqueous two-phase system optimised by RSM
Leanderson Tulio Marques Lemos1, Lorena Lucena de Medeiros2, Normando Ribeiro-Filho1
1Postgraduate Program in Food Science and Technology, Federal University of Paraíba, Campus I - Technology Center, CEP, 58051-900 João Pessoa, PB, Brazil; Postgraduate Program in Chemical Engineering, Federal University of Paraíba, Campus I - Technology Center, CEP, 58051-900 João Pessoa, PB, Brazil.
Abstract:
Xylitol is a sweetener, which can be produced by Candida yeasts. Xylitol production (XP) is expensive due to the high cost of enzymes production such as xylose reductase (XR). XR can be produced with a low-cost using cashew apple waste (CAW) hydrolysed; however, methods for XR purification are expensive. This study aims to optimise an ATPS using a response surface method (RSM) for xylose reductase (XR) purification. CAW was hydrolysed. Fermentations were conducted using yeast (CCT 1516) for XR production. Then, a RSM varying ATPS composition (polyetilenglycol (PEG, 1.6-2.4 g/L) and potassium phosphate (PP, 1-1.3 g/L) was applied for XR purification. The highest concentration of xylitol (15.4 g/L) generated was achieved at 40 h. The highest XR activity (0.4 U/mg) was reached at 28 h. RSM reveals that XR purification is positively affected by PEG, in contrast, PP did not affect XR purification.
Related Concept Videos
Microbes in Food Production
Microbes in Beverage Production
Bioreactor Controls-II
Bioreactor Controls-III
Production of Organic Acids

