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Updated: Sep 19, 2025

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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Storage protocol widely adopted for freshly collected soil samples significantly changes microbial sequencing and
Zhihao Chen1, Yonghong Wu2, Jan Dolfing3
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 211135, China; Zigui Three Gorges Reservoir Ecosystem, Observation and Research Station of Ministry of Water Resources, Yichang 443605, China; University of Chinese Academy of Sciences, Beijing 100049, China; University of Chinese Academy of Sciences, Nanjing 211135, China.
Science Bulletin
|June 15, 2025
Summary
No abstract available in PubMed .

