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Updated: Sep 19, 2025

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SCO-ph: Microfluidic Dynamic Phenotyping Platform for High-Throughput Screening of Single Cell Acidification.

Hyejoong Jeong1,2, Emilia A Leyes Porello1, Jean G Rosario2

  • 1Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|June 16, 2025
PubMed
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This summary is machine-generated.

This study introduces a microfluidic system for single-cell optical pH analysis (SCO-pH) to measure cellular metabolism. The high-throughput SCO-pH system can differentiate cells based on their extracellular acidification dynamics.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Quantitative high-throughput metabolism assays are crucial for single-cell phenotyping dynamics.
  • Extracellular acidification is a key metabolic phenotype linked to cellular processes like tumorigenicity.

Purpose of the Study:

  • To develop a versatile microfluidic system for single-cell optical pH analysis (SCO-pH).
  • To enable high-throughput, temporal extracellular pH analysis for dynamic single-cell phenotyping.

Main Methods:

  • Compartmentalization of single cells in 140-pL droplets within a microfluidic system.
  • Immobilization of approximately 40,000 droplets in a 2D array for analysis.
  • Encapsulation of a cell-impermeable pH probe for fluorescence-based pH quantification within each droplet.
Keywords:
extracellular acidificationglycolysis, high‐throughputmicrofluidicsphenotypingsingle cell

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Main Results:

  • The SCO-pH system successfully distinguished cells undergoing hyperglycolysis from untreated cells by monitoring extracellular acidification.
  • Single-cell heterogeneity in extracellular acidification dynamics was observed.
  • The system demonstrated the ability to differentiate cells based on metabolic activity.

Conclusions:

  • The developed SCO-pH system provides a high-throughput method for dynamic single-cell phenotyping.
  • This technology is valuable for applications requiring the analysis of cellular metabolism and heterogeneity.
  • SCO-pH facilitates the study of cellular metabolism and its implications in various biological contexts.