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Updated: Sep 19, 2025

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

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Humidity-Resistant Pt/CrVN₂ Fuel Cell Sensor for H₂S Biomarker Detection.

Zhaorui Zhang^1,2, Chonghui Zhu¹, Yu Liang¹

¹School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.

|June 16, 2025

Summary

This study introduces a novel sensor for detecting hydrogen sulfide (H₂S) biomarkers, even in humid conditions. The developed sensor offers sensitive, real-time monitoring for advanced medical diagnostics.

Keywords:

biomarker monitoring exhaled breath monitoring fuel cell gas sensor humidity-resistant sensing hydrogen sulfide (H2S) detection

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Area of Science:

Materials Science
Electrochemistry
Biomedical Engineering

Background:

Real-time biomarker monitoring is crucial for personalized diagnostics.
Hydrogen sulfide (H₂S) is a key biomarker for oral diseases.
Detecting low concentrations of H₂S in humid environments is challenging.

Purpose of the Study:

To develop a highly sensitive and humidity-resistant H₂S sensor.
To enable real-time, remote monitoring of H₂S biomarkers.
To create a practical system for breath diagnosis in smart healthcare.

Main Methods:

Fabrication of a platinum-loaded chromium vanadium nitride (Pt/CrVN₂) fuel cell-type H₂S sensor.
Evaluation of sensor performance, including detection limits, response time, and selectivity.
Testing sensor stability across a wide range of relative humidity (9%-79%).
Development and validation of a wireless detection system for breath analysis.

Main Results:

The Pt/CrVN₂ sensor achieved ultralow detection limits (50 ppb) with rapid response (4 s).
The sensor demonstrated exceptional selectivity and high resistance to humidity.
Stable performance was maintained across a broad humidity range (9%-79%).
A wireless system was successfully validated for practical breath diagnosis.

Conclusions:

The Pt/CrVN₂ sensor is a promising platform for sensitive, real-time biomarker detection.
This technology advances next-generation medical diagnostics, particularly in smart healthcare.
The sensor's humidity resistance overcomes a significant challenge in biomarker monitoring.