Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Colors and Magnetism03:02

Colors and Magnetism

12.4K
Color in Coordination Complexes
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human...
12.4K
The Pauli Exclusion Principle03:06

The Pauli Exclusion Principle

51.1K
The arrangement of electrons in the orbitals of an atom is called its electron configuration. We describe an electron configuration with a symbol that contains three pieces of information:
51.1K
Protein Folding01:22

Protein Folding

121.9K
Overview
121.9K
Atomic Nuclei: Nuclear Spin State Overview01:03

Atomic Nuclei: Nuclear Spin State Overview

1.2K
NMR-active nuclei have energy levels called 'spin states' that are associated with the orientations of their nuclear magnetic moments. In the absence of a magnetic field, the nuclear magnetic moments are randomly oriented, and the spin states are degenerate. When an external magnetic field is applied, the spin states have only 2 + 1 orientations available to them. A proton with = ½ has two available orientations. Similarly, for a quadrupolar nucleus with a nuclear spin value of...
1.2K
Atomic Nuclei: Nuclear Spin01:08

Atomic Nuclei: Nuclear Spin

3.2K
All atomic particles possess an intrinsic angular momentum, or 'spin'. Electrons, protons, and neutrons each have a spin value of ½, although protons and neutrons in nuclei may have higher half-integer spins owing to energetic factors.
Atomic nuclei have a net nuclear spin, , which can have an integer or half-integer value. In atomic nuclei, the spins of protons are paired against each other but not with neutrons, and vice versa. Consequently, an even number of protons does not...
3.2K
Spin–Spin Coupling: One-Bond Coupling01:17

Spin–Spin Coupling: One-Bond Coupling

1.1K
Coupling interactions are strongest between NMR-active nuclei bonded to each other, where spin information can be transmitted directly through the pair of bonding electrons. While nuclei polarize their electrons to the opposite spins, the bonding electron pair has opposite spins. Configurations with antiparallel nuclear spins are expected to be lower in energy. When coupling makes antiparallel states more favorable, J is considered to have a positive value. The one-bond coupling constant, 1J,...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chiral-Induced Spin Selectivity Effect in a 1 nm Thin 1,1'-Binaphthyl-2,2'-diyl Hydrogenphosphate Self-Assembled Monolayer on Nickel Oxide.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Chirality Induced Odd-Even Spin Selectivity in Self-Assembled Peptide-Based Helical Nanofibers.

ACS nano·2026
Same author

Chirality induced long-range spin-selective transport in helical 3D metal-organic frameworks.

Chemical science·2026
Same author

Dynamic breaking of mirror symmetry in spin-dependent electron transport through chiral media causes enantiomeric excesses.

Science advances·2026
Same author

Why Is the Mechanism Underlying the Chiral-Induced Selectivity Effect Still Challenging?

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Sevoflurane and Desflurane Spin-Decoherence Effect on Fe(III)acetylacetonate Redox Process.

Molecules (Basel, Switzerland)·2025
Same journal

Linking Local Water Electrostatic Potentials to Measured Hydrogen Evolution Onset in Aqueous Electrolytes.

The journal of physical chemistry letters·2026
Same journal

Microsolvation Redirects Electron-Induced Chemistry in Nucleobases.

The journal of physical chemistry letters·2026
Same journal

Interfacial Microenvironment Effects on the Mechanism of Photocatalytic Methanol Conversion for Hydrogen Evolution.

The journal of physical chemistry letters·2026
Same journal

Noncovalent Interactions in Protein-Ti Binding: Titan Bonds at Work.

The journal of physical chemistry letters·2026
Same journal

Partial Phase Remixing of Segregated Mixed Halide Perovskite Nanocrystals Induced by an Instant Change in an External Electric Field.

The journal of physical chemistry letters·2026
Same journal

Pressure-Driven Dissociation of a Kr Clathrate in the Presence of Colloids.

The journal of physical chemistry letters·2026
See all related articles

Related Experiment Video

Updated: Sep 19, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.7K

Protein Conformation Governs Spin-Selective Electron Transmission.

Naupada Preeyanka1, Tapan Kumar Das1, Ron Naaman1

  • 1Department of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 7610001, Israel.

The Journal of Physical Chemistry Letters
|June 17, 2025
PubMed
Summary
This summary is machine-generated.

The chiral induced spin selectivity (CISS) effect in proteins is significantly reduced upon denaturation. This highlights the crucial role of protein secondary structure in maintaining spin polarization for biological systems.

More Related Videos

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.1K

Related Experiment Videos

Last Updated: Sep 19, 2025

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
11:19

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels

Published on: July 4, 2016

10.7K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

1.9K
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

18.1K

Area of Science:

  • Biophysics
  • Materials Science
  • Biochemistry

Background:

  • Chiral induced spin selectivity (CISS) describes spin-dependent electron transport in chiral molecules.
  • Proteins possess chirality in their primary (amino acids) and secondary (helical structures) conformations.
  • Understanding the contribution of each chirality type to CISS in biological systems is crucial.

Purpose of the Study:

  • To investigate the impact of protein denaturation on spin polarization.
  • To differentiate the roles of primary and secondary structures in chiral spin selectivity.
  • To use d-glucose oxidase (GOx) as a model system to study these effects.

Main Methods:

  • Comparison of spin selective behavior of GOx in native and thermally denatured states.
  • Utilizing Hall-effect and magnetoresistance (MR) measurements.
  • Thermal denaturation at 65 °C and 95 °C.

Main Results:

  • Native GOx exhibits strong spin polarization, linked to its helical structure and FAD cofactor.
  • Thermal denaturation significantly reduces spin polarization, evidenced by decreased Hall voltage slope and MR values.
  • Loss of spin polarization correlates with the disruption of the protein's secondary structure.

Conclusions:

  • Protein secondary structure is essential for maintaining chiral potential landscapes and high spin polarization.
  • Denaturation disrupts secondary structure, leading to a loss of spin selectivity.
  • Spin-related structural properties remain even when the protein is integrated into a device.