Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.3K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
14.3K
Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A reagent-free binding assay for cholera toxin based on sequestration electrochemistry using lactose-functionalized magnetic microbeads.

The Analyst·2026
Same author

Surface-Modified Nickel Foam Cathodes for the Electroflotation of Multi-Component Batik Wastewater.

ACS omega·2026
Same author

Genetic features of Japanese children with ABCA3 deficiency.

Early human development·2026
Same author

The origins of noise in the Zeeman splitting of spin qubits in natural-silicon devices.

NPJ quantum information·2026
Same author

Novel Method for Characterizing Humic Substances Using Fluorescent Solvatochromism.

Sensors (Basel, Switzerland)·2026
Same author

Diagnosis of a Neonate With Long QT Syndrome and Severe Complications Delayed due to an Unrecognized Familial History.

Case reports in pediatrics·2025

Related Experiment Video

Updated: Sep 19, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.8K

Cell Sensing via a Peptide/Single-Strand DNA Probe-Modified Au Screen-Printed Electrode.

Kazuharu Sugawara1, Kenta Takeda1, Hideki Kuramitz2

  • 1Department of Life Engineering, Maebashi Institute of Technology, Gunma, 371-0816, Japan.

Chembiochem : a European Journal of Chemical Biology
|June 17, 2025
PubMed
Summary
This summary is machine-generated.

A novel single-strand DNA probe detects cancer cells using electron transfer and His-tag peptides. This method accurately quantifies K562 leukemia cells with high sensitivity and recovery in biological samples.

Keywords:
K562 cellscell sensingelectron‐transfer peptidesscreen‐printed electrodessingle‐strand DNA

More Related Videos

Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.5K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

18.6K

Related Experiment Videos

Last Updated: Sep 19, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

33.8K
Bacterial Detection & Identification Using Electrochemical Sensors
09:30

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

28.5K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

18.6K

Area of Science:

  • Biosensors and electrochemical detection
  • Cancer cell diagnostics
  • Nucleic acid aptamer technology

Background:

  • Early and accurate cancer cell detection is crucial for effective treatment.
  • Existing methods for cancer cell detection can be complex and time-consuming.
  • Development of sensitive and specific biosensors is needed for clinical applications.

Purpose of the Study:

  • To design and synthesize a novel single-strand DNA (ssDNA) probe for cancer cell detection.
  • To immobilize the probe onto an Au screen-printed electrode (AuSPE) for electrochemical sensing.
  • To evaluate the probe's performance in detecting human myeloid leukemia cells (K562).

Main Methods:

  • Design of an ssDNA probe incorporating an electron-transfer peptide and His-tag for sensing and purification.
  • Conjugation of a KK1B10 aptamer with a peptide sequence for target cell recognition.
  • Immobilization of the synthesized probe onto an AuSPE and electrochemical analysis of K562 cell interactions.

Main Results:

  • The optimized probe, Ac-CYYCYYCH6-AmC6-KK1B10 aptamer, demonstrated superior performance.
  • Electrode response decreased proportionally with increasing K562 cell concentrations.
  • A wide detection range (5-200 cells/mL) with a low detection limit (2 cells/mL) was achieved.
  • High recovery rates (99%-102%) were observed in human serum and bovine blood samples.

Conclusions:

  • The developed ssDNA probe-based electrochemical sensor is highly sensitive and specific for cancer cell detection.
  • The method shows promise for the rapid and accurate quantification of leukemia cells in complex biological matrices.
  • This approach offers a viable platform for developing advanced diagnostic tools for cancer.