Reference genome and reproduction-focused transcriptome for the threatened alpine tree frog ( Litoria verreauxii...

The alpine tree frog ( Litoria verreauxii alpina) is a threatened species found only above 1,200 meters within the Australian Alps. This species' distribution has been severely limited due to the pathogentic amphibian chytrid fungus, and current populations persist by recruitment. Here, we provide the first publicly available genome for the genus. We used PacBio HiFi reads as well as Hi-C scaffolding data to construct a high-quality genome. We also generated a reproduction focused transcriptome from brain, liver, and gonad tissues. The genome was 2.77 Gb in length and consisted of 962 contigs with a contig N50 of 37.2 Mb and an L50 of 19. This study provides the first publicly available reference genome for the Litoria genus to assist in conservation and reproduction focused works in amphibian management.