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Updated: Sep 19, 2025
Non-chromatographic Purification of Recombinant Elastin-like Polypeptides and their Fusions with Peptides and Proteins from Escherichia coli
Published on: June 9, 2014
Development of the Efficient Preparation Method for Thermoresponsive Elastin-Like Peptides Using Liquid-Phase
Keitaro Suyama1, Takeru Nose2,3
1Faculty of Arts and Science, Kyushu University, Fukuoka, Japan.
Abstract:
Elastin-like peptides (ELPs) are synthetic, repetitive sequences known for their temperature-dependent reversible self-assembly, making them environmentally friendly, biocompatible, and thermoresponsive. Despite various strategies for ELP production, the development of effective ELP supply methods remains a challenge. This chapter outlines a practical approach to synthesizing thermoresponsive ELPs using liquid-phase peptide synthesis (LPPS) with a hydrophobic benzyl alcohol support (HBA-Tag) and fragment condensation. This method effectively produces short ELPs that exhibit temperature-responsive self-assembly.
