New α-Pyrones From Endophytic Fusarium Sp. L46 Isolated From Dioscorea opposita.

Area of Science:

• Natural Product Chemistry
• Mycology
• Biochemistry

Background:

• Endophytic fungi, such as Fusarium sp. L46, residing within plants like Dioscorea opposita, are a rich source of novel bioactive metabolites.
• The exploration of endophytic fungi contributes to the discovery of new chemical entities with potential pharmacological applications.

Purpose of the Study:

• To isolate and characterize new and known compounds from the endophytic fungus Fusarium sp. L46.
• To evaluate the inhibitory potential of the isolated compounds against pancreatic lipase.

Main Methods:

• Isolation and purification of secondary metabolites using chromatographic techniques.
• Structure elucidation of new compounds employing 1D and 2D Nuclear Magnetic Resonance (NMR) spectroscopy and High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS).
• Assessment of pancreatic lipase inhibitory activity using established biochemical assays.

Main Results:

• Two new α-pyrone derivatives, fusarilactones D (1) and E (2), were identified.
• Four known compounds, phomapyrone C (3), fusarester C (4), neovasifuranone B (5), and neovasifuranone A (6), were also isolated and characterized.
• Compounds 3-5 were evaluated for their inhibitory effects on pancreatic lipase activity.

Conclusions:

• The endophytic fungus Fusarium sp. L46 produces a diverse array of α-pyrone derivatives.
• The isolated compounds, particularly phomapyrone C, fusarester C, and neovasifuranone B, demonstrate potential as pancreatic lipase inhibitors, warranting further investigation for therapeutic applications in metabolic disorders.