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Published on: June 13, 2021
Design, synthesis, and application of triazole-embedded maltose-neopentyl-glycol (TMNG) amphiphiles for membrane
Hongming Cai1, Xinyu Wu1, Shixin Jin1
1National Key Laboratory of Lead Druggability Research, Shanghai Institute of Pharmaceutical Industry Co., Ltd, China State Institute of Pharmaceutical Industry Co., Ltd., 285 Gebaini Road, Shanghai, 201203, China.
Abstract:
Understanding membrane protein (MP) structure and function is less advanced than that of soluble proteins. Detergents are the primary reagents used in membrane protein studies, yet only a limited selection is employed in both fundamental and applied research. This study presents the design, synthesis, and application of novel triazole-embedded maltose-neopentyl-glycol (TMNG) amphiphiles for membrane protein studies. Utilizing Copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC), the synthesis integrates two maltose hydrophilic subunits with the two-tailed lipid component, overcoming scalability issues encountered in previous methods. Thermal stabilization evaluations show that some TMNGs have equal or improved stabilizing effects compared to the widely used detergent maltose-neopentyl glycol (LMNG).

