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ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
Rapid detection of clenbuterol and ractopamine in pork: Sulfonate functionalized ion exchangemagnetic solid phase
Kaihan Yang1, Xiangben Yan2, Minmin Tang3
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Key Laboratory of Food Contact Materials Safety, State Administration for Market Regulation, Jiangsu Product Quality Testing & Inspection Institute, Nanjing, Jiangsu, 210007, China.
Abstract:
In order to achieve rapid and sensitive detection of clenbuterol (CLB) and ractopamine (RAC) in meat foods and ensure food safety, a magnetic nano-organic polymer (Fe3O4@SiO2@DVB-PSSNa) with π-π interaction, hydrogen bonding, and cation-exchange properties was synthesized for magnetic solid-phase extraction (MSPE) of CLB and RAC in pork. Coupled with a 96-well visual protein microarray chip, the method achieved LOD of 0.08 μg/kg (CLB) and 0.05 μg/kg (RAC), with recoveries of 87.4-101.9% (CLB) and 86.0-96.7% (RAC). The data indicates that the magnetic adsorbent demonstrates high efficiency in the enrichment and separation of analytes from complex substrates, suggesting its potential for routine rapid monitoring when integrated with visual protein microarray chips.
