Related Experiment Video
Updated: Sep 19, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Advancing nanopore technology toward protein identification and sequencing
1Department of Inorganic and Analytical Chemistry, Chemistry and Biochemistry, University of Geneva, 1211 Geneva, Switzerland; Institute of Bioengineering, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Abstract:
Proteins drive most cellular functions and are key players in diseases, yet proteomics still lags behind genomics due to the complexity, diversity, and dynamic nature of proteoforms. Nanopore technology - known for real-time, single-molecule DNA sequencing - is a promising contender to revolutionize protein analysis. The method has recently been adapted to proteins, showing strong potential for protein identification. However, true de novo protein sequencing with nanopores remains an open challenge. This review compares current nanopore-based strategies for protein analysis and highlights their technical hurdles towards application. Additionally, engineering strategies are explored aiming to bridge the gap towards single-molecule protein analysis and sequencing.
Related Concept Videos
RNA-seq
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Sanger Sequencing

