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Postbiotics: a perspective on their quantification.

Gabriel Vinderola1, Andrzej Benkowski2,3, Marion Bernardeau4

  • 1Instituto de Lactología Industrial (CONICET-UNL), Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santa Fe, Argentina.

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Summary
This summary is machine-generated.

Postbiotics, preparations of inanimate microbes conferring health benefits, require standardized quantification methods. This paper proposes guiding principles and a decision tree for characterizing these complex products.

Keywords:
International Scientific Association for Probiotics and Prebioticsflow cytometrymetabolitespostbioticsqPCRquantification

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Area of Science:

  • Microbiology
  • Biotechnology
  • Food Science

Background:

  • Postbiotics are defined as preparations of inanimate microorganisms or their components that provide health benefits.
  • Characterizing and quantifying postbiotics presents challenges due to their complex composition.
  • Existing methods lack a universal standard for postbiotic analysis.

Purpose of the Study:

  • To address challenges in postbiotic characterization and quantification.
  • To propose guiding principles for assessing postbiotic products.
  • To facilitate collaborative problem-solving in the postbiotic field.

Main Methods:

  • Discussion among academic and industry scientists at the ISAPP 2024 meeting.
  • Review of current and emerging quantification techniques.
  • Development of a decision tree for method selection.

Main Results:

  • Full characterization of all postbiotic components is often infeasible.
  • Essential elements for postbiotic description include progenitor microorganism identity/quantity and inactivation process.
  • Flow cytometry (FC) and quantitative polymerase chain reaction (qPCR) are valuable tools, but no single 'gold standard' exists.

Conclusions:

  • Quantification methods for postbiotics must be fit for purpose.
  • A decision tree is proposed to guide the selection of appropriate methods.
  • The proposed framework emphasizes microbial composition and provides guiding principles, acknowledging future technological advancements.