An improved preservation technique for cells of hemopoietic origin

A new technique for the cryopreservation of cell lines of hemopoietic origin is described. It uses a combination of 10% polyvinylpyrrolidone or hydroxyethylstarch with 15% glycerol, a slow stepwise addition of the cryoprotectants before freezing, cooling at 4 degrees C/min, rapid rewarming, and a slow step-wise dilution after thawing. This technique has given improved recovery rates with a number of basophil/mast cell lines, as well as a wide range of hemopoietic cells including hybridomas.