Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Outcomes with BCMA- and GPRC5D-Targeting Bispecific Antibodies in Plasma Cell Leukemia.

Blood advances·2026
Same author

Outcomes of acute myeloid leukemia patients admitted to the intensive care unit within 100 days of allogeneic hematopoietic stem cell transplantation.

The American journal of the medical sciences·2026
Same author

Fully human BCMA CAR-T therapy in multiple myeloma: lessons from long-term follow-up.

Translational cancer research·2026
Same author

Dexamethasone for Management of Cytokine Release Syndrome Associated With Talquetamab in Patients With Relapsed/Refractory Multiple Myeloma.

American journal of hematology·2026
Same author

Neighborhood Socioeconomic Disadvantage and Distance From Treatment Center do not Impact Survival Outcomes of Patients With Non-Hodgkin Lymphoma and Multiple Myeloma Treated With CAR T-Cell Therapies.

Transplantation and cellular therapy·2026
Same author

Multiple Myeloma Screening Education: A Community-Based Health Literacy Intervention.

Cancer research communications·2026

Related Experiment Video

Updated: Sep 18, 2025

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

4.2K

Signalling Network Analysis of Blood Mononuclear Cells From Clinical Samples by Bivariate Correlation.

David Kaplan1, Hillard M Lazarus1,2, Jason Valent3

  • 1CellPrint Biotechnology LLC, Shaker Heights, Ohio, USA.

Journal of Cellular and Molecular Medicine
|June 20, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new flow cytometry method to analyze cellular signaling networks in blood cells. The approach reveals cell-specific signaling patterns, offering insights into disease pathogenesis.

Keywords:
AL amyloidosisMGUSSTING pathwayapoptosismyelomaperipheral blood mononuclear cellsphosphoantigensplasma cell dyscrasiasignalling pathways

More Related Videos

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

58.0K
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

15.1K

Related Experiment Videos

Last Updated: Sep 18, 2025

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes
05:58

Evaluation of a Point-of-Care Testing Analyzer for Measuring Peripheral Blood Leukocytes

Published on: March 22, 2022

4.2K
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
09:12

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

58.0K
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

15.1K

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Assessing cellular signaling networks traditionally relies on analyzing individual phosphoantigens.
  • Existing methods may lack the resolution to fully delineate complex functional signaling networks in clinical samples.

Purpose of the Study:

  • To develop and validate a novel approach for analyzing functional signaling networks in blood cells using enhanced flow cytometry.
  • To apply this technology to identify cell-type specific signaling pathway features in patients with plasma cell disorders.

Main Methods:

  • Utilized enhanced flow cytometry with restricted-dimensional cytometry for increased resolving power and optimized analysis precision.
  • Validated the approach in short-term cultures by recapitulating known signaling pathway tenets.
  • Applied the technology platform to blood mononuclear cells from patients with plasma cell disorders.

Main Results:

  • Successfully recapitulated known signaling pathways, validating the novel approach.
  • Identified distinct patterns of bivariate correlations, revealing cell-type specific features of signaling pathways.
  • Provided nuanced views of signaling circuits and intermolecular relationships between signaling analytes.

Conclusions:

  • The developed flow cytometry approach enables detailed analysis of signaling networks in clinical blood samples.
  • This method can characterize cell-type specific signaling networks and their potential impact on pathophysiology and pathogenesis.
  • The findings offer a new platform for understanding disease mechanisms at the molecular signaling level.