Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Nuclei: Types of Nuclear Relaxation01:28

Atomic Nuclei: Types of Nuclear Relaxation

399
Nuclear relaxation restores the equilibrium population imbalance and can occur via spin–lattice or spin–spin mechanisms, which are first-order exponential decay processes.
In spin–lattice or longitudinal relaxation, the excited spins exchange energy with the surrounding lattice as they return to the lower energy level. Among several mechanisms that contribute to spin–lattice relaxation, magnetic dipolar interactions are significant. Here, the excited nucleus transfers...
399
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

302
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
302
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

924
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
924
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

310
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
310
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

1.5K
In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of Line Width and Anisotropy in <i>C</i><sub>3</sub>/<i>C</i><sub>4</sub>-Symmetric Gd(III) Complexes.

Inorganic chemistry·2026
Same author

Metal-Dependent Effects and Crowding Robustness of Pseudomonas fluorescens Esterase I.

Chembiochem : a European journal of chemical biology·2026
Same author

Ultra-Wideline 2D Correlations Among Low-γ Species in Solid-State NMR via the Progressive Saturation of a Common Proton Reservoir.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

On the effects of hyperpolarized water-based dissolution on the solute and solvent <sup>1</sup>H NMR spectra of small molecules.

Physical chemistry chemical physics : PCCP·2026
Same author

A Dynamic NMR Lineshape Simulation Framework for Lipid Diffusion and Membrane Thinning in Bicelles and Nanodiscs.

The journal of physical chemistry. B·2026
Same author

A Dynamic NMR Lineshape Simulation Framework for Lipid Diffusion and Membrane Thinning in Bicelles and Nanodiscs.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Sep 18, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.4K

Toward Generalized Solution-State 1H DNP NMR via Particle-Mediated Cross-Relaxation.

Sungsool Wi1, Angeliki Giannouli2, Korin Butbul2

  • 1National High Magnetic Field Laboratory, Tallahassee, Florida 32304, United States.

The Journal of Physical Chemistry Letters
|June 20, 2025
PubMed
Summary

This study demonstrates a new method for boosting proton nuclear magnetic resonance (NMR) signals using dynamic nuclear polarization (DNP) on small liquid samples. The technique enhances signals by transferring polarization from solid particles to the liquid phase.

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.1K
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
11:57

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Published on: September 13, 2019

6.7K

Related Experiment Videos

Last Updated: Sep 18, 2025

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.4K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.1K
Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
11:57

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Published on: September 13, 2019

6.7K

Area of Science:

  • Magnetic Resonance Spectroscopy
  • Physical Chemistry
  • Materials Science

Background:

  • Enhancing low-volume liquid-phase 1H NMR signals at high magnetic fields is challenging.
  • Dynamic Nuclear Polarization (DNP) offers a route to signal enhancement but often requires cryogenic temperatures or specific sample conditions.
  • Current DNP methods may not be compatible with high-resolution NMR requirements or standard sample volumes.

Purpose of the Study:

  • To investigate a novel DNP-based approach for enhancing 1H NMR signals in small liquid volumes (100 μL scale).
  • To achieve signal enhancement at noncryogenic temperatures using particle-mediated polarization transfer.
  • To explore the feasibility of this method for high-resolution NMR applications.

Main Methods:

  • Dispersion of proton-rich, DNP-active BDPA-doped polystyrene (PS) microparticles in a liquid sample (heptane).
  • Solid-state DNP enhancement of PS protons at 14.1 T and 185-220 K using 395 GHz microwave irradiation.
  • Measurement of liquid-phase 1H NMR signal enhancement via cross-relaxation from polarized solid particles.

Main Results:

  • Achieved a 12-fold enhancement of PS proton polarization within 2 seconds.
  • Observed approximately 3-fold enhancement of heptane proton resonances after 6 seconds of DNP, maintaining narrow line widths (≤2 Hz).
  • Optimized conditions using deuterated PS/PS/BDPA powder suspended in perdeuterated heptane-d16 for best results.

Conclusions:

  • Demonstrated a viable particle-mediated DNP method for enhancing liquid-phase 1H NMR signals at high fields.
  • The approach allows for signal enhancement without compromising sample volume, high-resolution conditions, or multiscan averaging.
  • This technique offers a generalizable strategy for future DNP-enhanced NMR applications.