Related Experiment Video
Updated: Sep 18, 2025

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
Vibrio parahaemolyticus VtrB contributes to bile resistance and regulatory influences extend beyond the pathogenicity
Lijun Yuan1, Rong Gao1, Laiwang Hou1
1College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, People's Republic of China.
Abstract:
VtrB is a novel ToxR-like transcriptional regulatory protein located on the Vibrio parahaemolyticus pathogenicity island (Vp-PAI). It can be induced by bile to activate the Vp-PAI genes, thereby enhancing the cytotoxicity and enterotoxicity of V. parahaemolyticus. However, the molecular mechanism of VtrB in bile resistance has not been thoroughly examined. The purpose of this study was to investigate the global regulatory network of VtrB. V. parahaemolyticus strain with vtrB deletion (ΔvtrB) was established. The response to bile of the wild type and ΔvtrB strain suggested that VtrB contributed to bile resistance and supplied a survival advantage to V. parahaemolyticus in the presence of bile. Motility assay and static biofilm assay showed that VtrB negatively regulates V. parahaemolyticus motility and biofilm formation in a bile-dependent manner. The cytotoxicity assay showed that ΔvtrB exhibited a significant reduction in toxicity toward Caco-2 cells. RNA sequencing and qRT-PCR were performed, and functions of the differentially expressed genes (DEGs) were investigated. RNA sequencing identified 701 DEGs in the ΔvtrB strain, including 462 up-regulated genes and 239 down-regulated genes. Functional enrichment analysis of DEGs indicated that VtrB contributes to bile resistance of V. parahaemolyticus by altering genes related to "Bacterial secretion system", "Flagella assembly and chemotaxis", "Biofilm formation", "Two-component system", "Quorum sensing", "Glyoxylate and dicarboxylate metabolism", "Citrate cycle", and "Carbon metabolism". Our findings suggested that VtrB may be part of a complex transcriptional network that regulates the expression of virulence genes and multiple cellular phenotypes.
More Related Videos
Related Concept Videos
Bacterial Translocation and Protein Secretion
Bacterial Phylum Verrucomicrobiota
Stringent Response in E. coli
Bacterial Phylum Proteobacteria
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

