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Related Experiment Video

Updated: Sep 18, 2025

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Effects of PIK3CA Gene Modifications on Radiosensitivity in Colorectal Cancer Cells.

Ghazi Alsbeih1, Khaled Al-Hadyan1

  • 1Radiation Biology Section, Biomedical Physics Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Asian Pacific Journal of Cancer Prevention : APJCP
|June 21, 2025
PubMed
Summary

Alterations in the PIK3CA gene impact radiosensitivity in colorectal cancer. PIK3CA amplification may increase radiation sensitivity, while mutations suggest radioresistance, guiding personalized radiotherapy.

Keywords:
PIK3CARadiosensitivitycolorectal cancergene amplificationgene mutation

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Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy Research

Background:

  • The phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) is crucial for cell growth and survival.
  • PIK3CA mutations are implicated in the PIK3CA-related overgrowth spectrum (PROS) and various cancers, including colorectal cancer.
  • The precise impact of PIK3CA alterations on radiotherapy response remains largely unelucidated.

Purpose of the Study:

  • To investigate the association between PIK3CA gene modifications (amplification and mutation) and radiosensitivity in colorectal cancer cell lines.
  • To explore the potential of PIK3CA alterations as predictive biomarkers for radiotherapy response.

Main Methods:

  • Analysis of 8 established colorectal cancer cell lines (HT-29, DLD-1, HCT-116, SW480, HCT-15, Colo-320, and LoVo).
  • Assessment of PIK3CA gene amplification and mutation status.
  • Correlation of genetic alterations with observed radiosensitivity phenotypes.

Main Results:

  • A statistically significant association (p < 0.05) was found between PIK3CA modifications and radiosensitivity in colorectal cancer cells.
  • PIK3CA gene amplification (> 2) correlated with increased sensitivity to radiation.
  • PIK3CA mutations were associated with increased radioresistance in colorectal cancer cells.

Conclusions:

  • PIK3CA gene alterations exhibit distinct effects on radiosensitivity in colorectal cancer.
  • PIK3CA amplification and mutations may serve as potential biomarkers for personalized radiotherapy selection.
  • Further research is warranted to validate these preliminary findings and elucidate the underlying mechanisms for tailored cancer treatment.