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Published on: April 29, 2016
Protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model
Shani Talice1, Shany K Barkan1, Grace A Snyder2
1The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Center for Regenerative Medicine and Stem Cells, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel; The Goldman Sonnenfeldt School of Sustainability and Climate Change, Ben Gurion University of the Negev, Beer Sheva 8410501, Israel.
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Stem cell transplantation is a promising strategy for regenerating damaged tissues in Hexacorallia, a cnidarian subclass that includes stony corals threatened by anthropogenic stressors. Here, we present a protocol for cell transplantation in Nematostella vectensis, a Hexacorallian model. We describe steps for chemotherapy treatment, cell dissociation, and transplantation. We then detail procedures for assessment via fluorescence microscopy and flow cytometry. This technique lays the groundwork for potential coral restoration and conservation applications. For complete details on the use and execution of this protocol, please refer to Talice et al.1.
