Jove
Visualize
Contact Us

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acute sublethal ammonia exposure suppresses neurotransmitter expression and impairs behaviors in the early development stages of zebrafish.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026
Same author

Rapid prenatal CNV detection using nanopore technology.

BMC research notes·2026
Same author

HLA - C*03:02:02 and DPA1*01:03:01 protect against discoid rash in Thai Systemic Lupus Erythematosus patients.

Scientific reports·2026
Same author

Comparative Genetic Diversity and Population Structure of Wild <i>Atalantia</i> from Taiwan and Sri Lanka Using SSR Markers.

Plants (Basel, Switzerland)·2026
Same author

ALDH2 inhibits head and neck tumorigenesis through RAS signaling suppression, transactivation of TGM2, and synergy with ALDH6A1.

Cellular and molecular life sciences : CMLS·2026
Same author

A case with a de novo chromosome 8.9 Mb 11pter duplication and 6.4 Mb 11qter deletion derived from a father with a normal karyotype.

Clinical dysmorphology·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 16, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

13.1K

Protocol for spatial analysis of multiple markers across adjacent tissue sections captured by RNAscope using

Katherine Copeland1, Ariestya Indah Permata Sari2, Justin Copeland1

  • 1Science Division, Mahidol University International College, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.

STAR Protocols
|June 22, 2025
PubMed
Summary

This study introduces a new protocol for quantifying gene expression in single cells using advanced imaging analysis. The method aids in comparing gene expression between metastatic and non-metastatic cancer samples.

Keywords:
BioinformaticsCancerCell BiologyGene ExpressionIn Situ HybridizationSingle CellSystems biology

More Related Videos

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.8K

Related Experiment Videos

Last Updated: Jul 16, 2026

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

13.1K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.8K

Area of Science:

  • Biomedical Imaging
  • Molecular Biology
  • Cancer Research

Background:

  • Accurate quantification of gene expression at single-cell resolution is crucial for understanding complex biological processes.
  • Distinguishing gene expression patterns in metastatic versus non-metastatic cancer is key for targeted therapies.

Purpose of the Study:

  • To present a detailed protocol for quantifying gene expression using fluorescence in situ hybridization (FISH) at single-cell spatial resolution.
  • To enable comparative analysis of gene expression between cancer samples with and without metastasis.

Main Methods:

  • Utilizing an imaging analysis pipeline based on CellProfiler software for gene expression quantification.
  • Employing RNAscope multiplex fluorescent assays for target transcript detection.
  • Leveraging Vectra Polaris Automated Quantitative Pathology Imaging for visualization.
  • Offering an option for analyzing signal interactions across adjacent tissue sections.

Main Results:

  • The protocol provides a standardized method for high-resolution gene expression analysis.
  • Facilitates the identification of differential gene expression patterns associated with metastasis.
  • Enables spatial analysis of gene expression and transcript interactions within tissue microenvironments.

Conclusions:

  • This protocol offers a robust framework for single-cell spatial gene expression analysis.
  • It serves as a valuable tool for cancer research, particularly in understanding metastasis.
  • The method can be adapted for various biological investigations requiring precise spatial transcriptomic data.