Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Acetylcholine responsiveness of the BetI regulatory system in Escherichia coli.

The Journal of general and applied microbiology·2026
Same author

Estimating thresholds for walking independence after stroke: a comparison of item response and classical test theories.

Physiotherapy theory and practice·2026
Same author

A Choline-Responsive Band-Pass gene circuit without transcriptional cascades.

The Journal of general and applied microbiology·2026
Same author

Ramsay Hunt syndrome with atypical auricular findings: Reports of two cases.

Pediatrics international : official journal of the Japan Pediatric Society·2026
Same author

As(III)-induced protein aggregation: insights and emerging possibilities for As(III)-responsive proteins.

Bioscience, biotechnology, and biochemistry·2025
Same author

[A Case of Interstitial Lung Disease That Developed after the First Administration of Trastuzumab-Deruxtecan in a Patient with Recurrent Breast Cancer].

Gan to kagaku ryoho. Cancer & chemotherapy·2025
Same journal

Bacillus subtilis var. natto as a naturally optimized biotin biosensor.

The Journal of general and applied microbiology·2026
Same journal

FAS2-1280F mutation in the fatty acid synthase gene, FAS2, of Saccharomyces cerevisiae enhances caprylic acid production.

The Journal of general and applied microbiology·2026
Same journal

Biotin Dependency Shapes Sporulation Kinetics in Bacillus subtilis var. natto.

The Journal of general and applied microbiology·2026
Same journal

Reversible dissociation of peripheral subunits from the photosynthetic reaction center of the green sulfur bacterium Chlorobaculum tepidum.

The Journal of general and applied microbiology·2026
Same journal

Betaine and choline are important compatible solutes in Bacillus altitudinis under salt and glucose stress.

The Journal of general and applied microbiology·2026
See all related articles

Related Experiment Video

Updated: Sep 18, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K

Screening system for MAA-CoA productivity using 2-methylcitrate biosensor.

Satoshi Hasegawa1, Ryota Kato1, Daichi Ishihara1

  • 1Department of Applied Chemistry, Faculty of Science, and Engineering, Waseda University.

The Journal of General and Applied Microbiology
|June 22, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a biosensor to measure methacrylyl-CoA (MAA-CoA) levels, enabling evolutionary engineering for a more feasible biosynthetic route to methyl methacrylate (MMA). This system aids in optimizing MMA production from isobutyric acid.

Keywords:
2-methylcitrateEscherichia coligenetically-encoded biosensormetabolic engineeringmethyl methacrylate

More Related Videos

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.1K

Related Experiment Videos

Last Updated: Sep 18, 2025

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
08:10

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

8.9K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
10:21

A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries

Published on: February 1, 2011

16.1K

Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Synthetic Biology

Background:

  • Methyl methacrylate (MMA) is a key monomer for acrylic resins, with growing demand and recycling potential.
  • Current biosynthetic routes for MMA face challenges due to the toxicity and instability of intermediates like methacrylyl-CoA (MAA-CoA) and low enzyme activity.
  • Developing efficient and sustainable production methods for MMA is crucial.

Purpose of the Study:

  • To construct a high-throughput biosensor system for quantifying cellular methacrylyl-CoA (MAA-CoA) levels.
  • To facilitate evolutionary engineering of the MMA biosynthetic pathway and its enzymatic components.
  • To enable the optimization and breeding of a novel MMA production pathway.

Main Methods:

  • Development of a novel biosensor for detecting methacrylyl-CoA (MAA-CoA) in cellular environments.
  • Utilization of the biosensor in a high-throughput screening manner.
  • Establishment of a functional metabolic pathway from isobutyric acid to MAA-CoA using the biosensor system.

Main Results:

  • A functional biosensor system for evaluating methacrylyl-CoA (MAA-CoA) cellular levels was successfully constructed.
  • The biosensor enabled the establishment of a complete biosynthetic pathway from isobutyric acid to MAA-CoA.
  • This system supports the design-build-test-learn cycle for pathway optimization.

Conclusions:

  • The developed MAA-CoA biosensor is a valuable tool for advancing the evolutionary engineering of MMA biosynthetic pathways.
  • This work paves the way for more feasible and efficient microbial production of methyl methacrylate.
  • The biosensor system can accelerate the optimization and breeding of MMA production strains.