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Updated: Sep 18, 2025
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
Effect of Syk Inhibitory Activity of Vialinin A on Degranulation from Antigen-Stimulated RBL-2H3 Cells
Ange Murielle Djidjou Tagne1, Kouichi Sugaya1, Jun-Ichi Onose1
1Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Abstract:
As previously reported, vialinin A, inhibits tumor necrosis factor α (TNF-α) production and extracellular release from RBL-2H3 cells by targeting USP5. In this study, we examined its inhibitory effect and mechanism of action on immunoglobulin E (IgE)-mediated early-phase allergic reactions in RBL-2H3 cells. Vialinin A inhibited β-hexosaminidase release from RBL-2H3 cells and tended to suppress cytosolic calcium ion concentration elevations. Kinase analysis and results from immunoblotting showed that vialinin A inhibited Syk activation and its autophosphorylation. Consequently, the phosphorylation of Syk downstream signalling proteins, such as linker for activation of T cells family and phospholipase Cγ1/2, essential in calcium ion mobilization and cell activation were slightly inhibited. These results suggest that vialinin A affects degranulation of RBL-2H3 cells by inhibiting Syk.
