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Published on: January 17, 2012
Vialinin A Inhibits Microtubule-Mediated Secretory Granule Transport for Degranulation in RBL-2H3 Cells by Targeting
Ange Murielle Djidjou Tagne1, Kotoe Ishii1, Yasukiyo Yoshioka2
1Department of Nutritional Science and Food Safety, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Abstract:
The bioregulatory function of food-derived bioactive factors in complementary therapies for lifestyle-related diseases is attracting worldwide attention. Vialinin A, a p-terphenyl compound isolated from the edible Chinese mushroom Thelephora vialis, inhibits the production and release of tumor necrosis factor α and Syk kinase activity, a key protein involved in the development of allergic rhinitis. However, its effect on other calcium-independent signaling molecules remains unclear. This study investigated the effects of vialinin A on microtubule formation signaling pathways in RBL-2H3 cells. The results showed that vialinin A inhibited Fyn kinase activity and reduced PI3K (class IA) phosphorylation in a dose-dependent manner. Notably, vialinin A inhibited PI3Kδ kinase activity and reduces Akt phosphorylation even at low concentrations. These findings suggest that vialinin A suppresses microtubule formation and granule translocation required for mast cell degranulation through inhibition of the Fyn-PI3K-Akt signaling pathway. Collectively, vialinin A may represent a promising lead compound for the development of anti-allergic therapeutics.
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