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Cytotoxicity of chitosan-loaded triple antibiotic paste versus conventional triple antibiotic paste on dental pulp
Asiya Sameer Mujawar1, Varsha Pandit1, Sumaiyya Shaikh2
1Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth Deemed to be University Dental College and Hospital, Pune, Maharashtra, India.
Background:
Triple antibiotic paste (TAP) is a commonly used intracanal medicament in regenerative endodontics. However, its cytotoxic effects on dental pulp stem cells (DPSCs) remain a concern. Incorporating chitosan into TAP may enhance its biocompatibility while retaining antimicrobial properties.
Aim:
The aim of this study was to evaluate and compare the cytotoxicity of chitosan-loaded TAP (Ch-TAP) with conventional TAP on DPSCs at varying concentrations and time intervals.
Materials And Methods:
DPSCs were exposed to Ch-TAP and TAP at concentrations of 0.1-100. Cell viability was assessed at 1-4 weeks using an MTT assay. The results were statistically analyzed using one-way ANOVA and post hoc Tukey's test to identify differences in cytotoxic effects between groups.
Results:
Ch-TAP demonstrated superior cell viability at all concentrations compared to TAP. The highest cell viability was observed with Ch-TAP at 0.1 mg/mL, maintaining 96% viability at 1 week and 85% at 4 weeks. Higher concentrations of both pastes exhibited a dose dependent decrease in cell viability, with Ch-TAP consistently showing less cytotoxicity. TAP at 100 mg/mL exhibited the highest cytotoxicity, reducing viability to 20% by the 4th week.
Conclusion:
Ch-TAP showed significantly lower cytotoxicity and better biocompatibility with DPSCs compared to conventional TAP, particularly at lower concentrations.
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