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Updated: Sep 18, 2025

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

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Published on: June 18, 2020

Cellular Redox Regulation and Fluorescence Imaging.

Yong Li¹, Guangzhao Liu¹, Simiao Cheng¹

¹College of Chemistry, Chemical Engineering and Materials Science, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Institutes of Biomedical Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.

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|June 23, 2025

Summary

Cellular redox homeostasis is vital for biological processes and disease. This review covers biomolecules, regulatory mechanisms, and advanced fluorescent probes for imaging redox status in diseases.

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Last Updated: Sep 18, 2025

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Area of Science:

Biochemistry and Molecular Biology
Cellular Biology
Biomedical Imaging

Background:

Cellular redox homeostasis is critical for biological functions and implicated in diseases.
Dysregulation of redox status is a key pathogenic mechanism in inflammatory diseases, cancer, and neurodegenerative disorders.
Biomolecules and redox-active metals are involved in maintaining and indicating cellular redox status.

Purpose of the Study:

To review biomolecules involved in cellular redox maintenance and their regulatory mechanisms.
To systematically examine recent advancements in fluorescent probes for redox biomarker imaging.
To discuss the therapeutic and diagnostic potential of redox regulation and imaging.

Main Methods:

Literature review focusing on biomolecules regulating redox homeostasis.
Systematic review of fluorescent probes for redox biomarker detection (past five years).
Analysis of probe design, reaction mechanisms, and bioimaging applications.

Main Results:

Identified key biomolecules and regulatory pathways in cellular redox homeostasis.
Highlighted novel fluorescent probes with specific reaction mechanisms for redox imaging.
Demonstrated applications of these probes in visualizing redox changes in disease models.

Conclusions:

Cellular redox homeostasis is a crucial target for understanding and treating diseases.
Fluorescent probes offer powerful tools for in situ imaging of redox status.
Further research in redox regulation and imaging holds promise for diagnostics and therapeutics.