Related Experiment Video

Updated: Aug 12, 2026

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology
11:42

Solid Phase Synthesis of a Functionalized Bis-Peptide Using "Safety Catch" Methodology

Published on: May 15, 2012

Improved approach for anchoring N alpha-9-fluorenylmethyloxycarbonylamino acids as p-alkoxybenzyl esters in

F Albericio, G Barany

    International Journal of Peptide and Protein Research
    |July 1, 1985
    PubMed

    More Related Videos

    Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials08:55

    Facile Protocol for the Synthesis of Self-assembling Polyamine-based Peptide Amphiphiles (PPAs) and Related Biomaterials

    Published on: June 25, 2018

    An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis06:19

    An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

    Published on: November 22, 2024

    Related Concept Videos

    Preparation of Amines: Alkylation of Ammonia and Amines01:30

    Preparation of Amines: Alkylation of Ammonia and Amines

    Alkylation is one of the methods used to prepare amines. Direct alkylation of ammonia or a primary amine with an alkyl halide gives polyalkylated amines along with a quaternary ammonium salt through successive SN2 reactions. This process of making the quaternary salt through the direct alkylation method is called exhaustive alkylation.
    Each alkylation step makes the nitrogen center more nucleophilic, which triggers successive alkylations until a quaternary ammonium salt is formed. Considering...
    Preparation of 1° Amines: Azide Synthesis01:22

    Preparation of 1° Amines: Azide Synthesis

    Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
    Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
    Amines to Amides: Acylation of Amines01:19

    Amines to Amides: Acylation of Amines

    Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
    Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary amide...
    Preparation of 1° Amines: Gabriel Synthesis01:28

    Preparation of 1° Amines: Gabriel Synthesis

    Direct alkylation is not a suitable method for synthesizing amines because it produces polyalkylated products. Gabriel synthesis is the most preferred method to exclusively make primary amines. The method uses phthalimide, which contains a protected form of nitrogen that participates in alkylation only once to predominantly give primary amines.
    Strong bases like NaOH or KOH deprotonate the phthalimide to form the corresponding anion, which acts as a nucleophile. Further, the anion attacks an...
    Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis01:07

    Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis

    Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an alkylated β-keto acid.
    Alkylation of β-Diester Enolates: Malonic Ester Synthesis01:14

    Alkylation of β-Diester Enolates: Malonic Ester Synthesis

    Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.