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Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Sep 18, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Proximity Ligation Assay.

Shan Grewal^1,2, Shawn C Chafe^2,3, Chitra Venugopal^2,3

¹Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|June 24, 2025

Summary

This summary is machine-generated.

The Proximity Ligation Assay (PLA) visualizes protein interactions in tumor cells. This method detects protein colocalization in situ, offering insights into brain tumor progression.

Keywords:

Colocalization Fluorescence microscopy In situ assays Protein–protein interactions Spatial organization

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Last Updated: Sep 18, 2025

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

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Published on: June 9, 2017

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Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Protein-protein interactions are crucial for cellular functions.
Understanding these interactions is key to deciphering disease mechanisms.
The Proximity Ligation Assay (PLA) offers a sensitive method for detecting these interactions.

Purpose of the Study:

To detail a protocol for performing PLA in patient-derived tumor cells.
To visualize in situ protein colocalization using fluorescence microscopy.
To highlight PLA's utility in studying brain tumor progression.

Main Methods:

Utilizing PLA with DNA probes tethered to antibodies against target proteins.
Detecting protein complexes within a 40-nm proximity.
Employing rolling circle amplification for signal generation and fluorescence microscopy for detection.

Main Results:

Successful visualization of protein colocalization in patient-derived tumor cells.
Demonstration of PLA's high specificity and sensitivity in complex biological samples.
Accurate capture of protein-protein interactions and spatial organization within cells.

Conclusions:

PLA is a valuable tool for studying protein interactions in situ.
The described protocol facilitates the application of PLA in tumor cell research.
PLA provides critical insights into the molecular mechanisms of brain tumor progression.