Evaluation of the effects of various anti-arthritic drugs on type II collagen-induced mouse arthritis model
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Abstract:
A battery of drugs which are commonly used as therapeutic agents for arthritis was tested for effects on the inflammatory and immunological responses of DBA/1J mice, after immunization with type II collagen. All the drugs were tested at more than one dosage. The mice were protected from the development of arthritis by treatment with paramethasone (0.25 mg/kg/day) or cyclophosphamide (5 mg/kg/day). The nonsteroidal anti-inflammatory drugs used in these studies, viz. aspirin (200 mg/kg/day), benoxaprofen (100 mg/kg/day) and naproxen (200 mg/kg/day), had no significant effect on the joint involvement, although naproxen and benoxaprofen at these high doses caused some reduction of immune responses of mice to collagen. Chloroquine (100 mg/kg/day), levamisol (50 mg/kg/day) and gold chlorophosphene (5 mg/kg/day) had no effect on the inflammatory or humoral response, while treatment with D-penicillamine (100 mg/kg/day) led to an early onset of arthritis in mice. These data suggest that the type II collagen-induced mouse arthritis model may not be highly suitable for detection of the traditional nonsteroidal anti-inflammatory class of drugs or the anti-rheumatic drugs, although the possibility remains that some new and novel immunosuppressive agents may be detected with this model.
Insights
This study tested arthritis drugs on mice. Only paramethasone and cyclophosphamide prevented arthritis, suggesting the model is better for detecting novel immunosuppressants than traditional anti-inflammatory drugs.
Area of Science:
- Immunology
- Pharmacology
- Rheumatology
Background:
- Arthritis is a chronic inflammatory condition affecting joints.
- Type II collagen-induced arthritis in DBA/1J mice is a common preclinical model.
- Evaluating drug efficacy in this model is crucial for arthritis treatment development.
Purpose of the Study:
- To assess the effects of common arthritis therapeutic agents on inflammatory and immunological responses in a collagen-induced arthritis mouse model.
- To determine the suitability of this model for evaluating different classes of anti-arthritic drugs.
Main Methods:
- DBA/1J mice were immunized with type II collagen to induce arthritis.
- Mice were treated with various drugs including paramethasone, cyclophosphamide, aspirin, benoxaprofen, naproxen, chloroquine, levamisole, gold, and D-penicillamine at multiple dosages.
- Inflammatory and immunological responses, as well as joint involvement, were monitored.
Main Results:
- Paramethasone and cyclophosphamide significantly protected mice from arthritis development.
- Nonsteroidal anti-inflammatory drugs (aspirin, benoxaprofen, naproxen) showed no significant effect on joint inflammation.
- Naproxen and benoxaprofen partially reduced immune responses to collagen, while chloroquine, levamisole, and gold had no effect.
- D-penicillamine accelerated arthritis onset.
Conclusions:
- The type II collagen-induced mouse arthritis model may not be ideal for detecting traditional nonsteroidal anti-inflammatory drugs or general anti-rheumatic drugs.
- This model shows potential for identifying novel immunosuppressive agents for arthritis treatment.