Challenges in the development of microfluidic paper-based analytical devices (μPADs)

Area of Science:

• Analytical Chemistry
• Materials Science
• Biomedical Engineering

Background:

• Microfluidic paper-based analytical devices (μPADs) utilize capillary action to analyze small fluid volumes.
• The field, initiated in 2007, offers advantages for point-of-care testing (POCT).
• Paper's role in analytical chemistry has evolved from chromatography to advanced μPADs.

Purpose of the Study:

• To review the evolution of paper-based analytical devices.
• To examine challenges in μPAD development for reliable POCT.
• To present methods and applications for overcoming these challenges.

Main Methods:

• Literature review of paper-based analytical devices.
• Analysis of technological advancements in μPAD fabrication.
• Examination of real-world applications, particularly in health sciences.

Main Results:

• μPADs offer a low-cost, portable platform for diagnostics.
• Key challenges include fluid control, reproducibility, and integration for POCT.
• Solutions involve advanced fabrication techniques and material science innovations.

Conclusions:

• μPADs represent a significant advancement in analytical chemistry and POCT.
• Addressing current challenges is crucial for widespread adoption in healthcare.
• Future directions focus on enhancing device reliability and expanding application scope.