Advances in Gelatin-Based Tissue Engineering Using HRP/H₂O₂

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Polymer Chemistry

Background:

• Gelatin is a biocompatible polymer with tunable properties, making it valuable for tissue engineering.
• Hydrogel fabrication is crucial for tissue engineering applications.
• The horseradish peroxidase (HRP)/hydrogen peroxide (H2O2) system offers an effective method for creating modifiable gelatin-based hydrogels.

Purpose of the Study:

• To review recent advancements in using the HRP/H2O2 catalytic system for gelatin-based hydrogel fabrication.
• To emphasize the role of HRP/H2O2 in tailoring hydrogel properties for tissue engineering.
• To explore emerging trends like in situ gelation and hybrid bioinks for tissue engineering.

Main Methods:

• A comprehensive literature review was conducted using Scopus and Web of Science databases.
• Data extraction focused on materials, preparation methods, cell types, animal models, and computational techniques.
• Analysis included both homopolymeric and copolymeric hydrogels based on gelatin.

Main Results:

• The HRP/H2O2 system allows fine-tuning of gelatin hydrogel properties by adjusting catalyst concentrations.
• Variations in HRP and H2O2 concentrations significantly impact gel characteristics for specific tissue engineering needs.
• Emerging trends like in situ gelation and hybrid bioinks show promise, supported by cell culture and animal model data.

Conclusions:

• The HRP/H2O2 system is a powerful tool for creating versatile gelatin hydrogels for tissue engineering.
• Precise control over HRP/H2O2 concentrations is key to optimizing hydrogel performance.
• Future tissue engineering applications can benefit from in situ gelation and hybrid bioink strategies using these hydrogels.