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Advances in Gelatin-Based Tissue Engineering Using HRP/H2O2.

Marino Basha1, Ahmad Aburub1, Filippos F Karageorgos1

  • 1Department of Transplantation Surgery, Center for Research and Innovation in Solid Organ Transplantation, School of Medicine, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece.

Gels (Basel, Switzerland)
|June 25, 2025
PubMed
Summary
This summary is machine-generated.

Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) enable tunable gelatin hydrogels for tissue engineering. Optimizing HRP/H2O2 concentrations fine-tunes gel properties for diverse applications.

Keywords:
3D printinggelatinhorseradish peroxidasehydrogelhydrogen peroxideinterpenetrating networkmicrogelnanofiberstissue engineeringwound healing

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Gelatin is a biocompatible polymer with tunable properties, making it valuable for tissue engineering.
  • Hydrogel fabrication is crucial for tissue engineering applications.
  • The horseradish peroxidase (HRP)/hydrogen peroxide (H2O2) system offers an effective method for creating modifiable gelatin-based hydrogels.

Purpose of the Study:

  • To review recent advancements in using the HRP/H2O2 catalytic system for gelatin-based hydrogel fabrication.
  • To emphasize the role of HRP/H2O2 in tailoring hydrogel properties for tissue engineering.
  • To explore emerging trends like in situ gelation and hybrid bioinks for tissue engineering.

Main Methods:

  • A comprehensive literature review was conducted using Scopus and Web of Science databases.
  • Data extraction focused on materials, preparation methods, cell types, animal models, and computational techniques.
  • Analysis included both homopolymeric and copolymeric hydrogels based on gelatin.

Main Results:

  • The HRP/H2O2 system allows fine-tuning of gelatin hydrogel properties by adjusting catalyst concentrations.
  • Variations in HRP and H2O2 concentrations significantly impact gel characteristics for specific tissue engineering needs.
  • Emerging trends like in situ gelation and hybrid bioinks show promise, supported by cell culture and animal model data.

Conclusions:

  • The HRP/H2O2 system is a powerful tool for creating versatile gelatin hydrogels for tissue engineering.
  • Precise control over HRP/H2O2 concentrations is key to optimizing hydrogel performance.
  • Future tissue engineering applications can benefit from in situ gelation and hybrid bioink strategies using these hydrogels.