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Related Experiment Video

Updated: Sep 18, 2025

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A Minimally Invasive Transthoracic Injection Technique for Reproducible Intrapleural Delivery in Mice.

Sophie Rovers1, Pooyeh Farahmand2, Dana Liu1

  • 1Center for Oncological Research (CORE), University of Antwerp, 2000 Antwerpen, Belgium.

Methods and Protocols
|June 25, 2025
PubMed
Summary
This summary is machine-generated.

A new minimally invasive technique simplifies creating pleural mesothelioma (PM) models. This reliable method improves orthotopic model generation and animal welfare for better PM research.

Keywords:
intrapleural injectionminimally invasive methodsorthotopic modelspleural mesotheliomapreclinical modelstransthoracic injection

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Area of Science:

  • Preclinical research
  • Oncology
  • Thoracic surgery

Background:

  • Standardized and reproducible preclinical models are crucial for advancing pleural mesothelioma (PM) research.
  • Existing methods for generating orthotopic PM models can be complex and invasive.

Purpose of the Study:

  • To present a simple, reliable, and minimally invasive transthoracic intrapleural injection technique for improved orthotopic pleural mesothelioma model generation.
  • To reduce technical complexity, animal stress, and the need for surgery or general anesthesia.

Main Methods:

  • A transthoracic intrapleural injection technique using a needle sleeve to control injection depth.
  • The method avoids the need for surgery or general anesthesia, ensuring precise delivery into the pleural cavity.

Main Results:

  • Achieved a 100% tumor engraftment rate with AE17 tumor cells.
  • Successfully demonstrated versatility through asbestos fiber injection in mesothelioma models.

Conclusions:

  • This technique offers a more accessible and standardized alternative for generating reliable pleural mesothelioma models.
  • The improved reliability and ease of adoption can facilitate broader research, even for those with limited surgical experience.