Lactobacillus Supplementation Modulates Rumen Microbiota and Metabolism in Yaks Under Fattening Feeding Conditions: A...

Area of Science:

• Rumen microbiology
• Animal nutrition
• Metabolomics

Background:

• The rumen's role in nutrient digestion is vital for ruminants.
• Mechanisms of Lactobacillus modulation of rumen homeostasis under high-energy diets are unclear.

Purpose of the Study:

• Investigate Lactobacillus's regulatory effects on yak rumen microbiota and metabolism.
• Assess impacts on rumen structure and function under concentrate-based rearing.

Main Methods:

• Multi-omics analyses: rumen microbial sequencing and metabolomics.
• Correlation analysis of phenotypic data in 80 male Pamir yaks over 170 days.
• Lactobacillus intervention in a high-energy diet model.

Main Results:

• High-energy diets negatively impacted yak rumen histomorphology and microbiota.
• Lactobacillus optimized rumen microbiome: promoted mucous layer, reduced Prevotella/Ruminococcus, increased Fibrobacter/Muribaculaceae.
• Upregulated fatty acid metabolism pathways and altered metabolite concentrations.

Conclusions:

• Lactobacillus supplementation benefits yak ruminal health under high-energy feeding.
• Optimized rumen microbiome and metabolism support intensive rearing without compromise.
• Regulated metabolites are potential biomarkers for Lactobacillus intervention efficacy.