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Transcriptomic Analysis of Tambaqui (Colossoma macropomum) Exposed to Trichlorfon-Induced Toxicity
Hallana Cristina Menezes da Silva1, Igor Kelvyn Cavalcante Lobo2, André Gentil da Silva3
1Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisas da Amazônia, Manaus 69067-375, AM, Brazil.
Animals : an Open Access Journal From MDPI
|June 26, 2025
Summary
This study reveals how the antiparasitic trichlorfon affects tambaqui fish liver cells. Exposure activates pathways linked to tumors, immunity, and cell death, with altered gene expression impacting fish health in aquaculture.
Area of Science:
- Aquatic toxicology
- Molecular biology
- Fish health
Background:
- Trichlorfon is a common antiparasitic in aquaculture.
- Tambaqui (C. macropomum) is a key farmed fish species in the Amazon.
- Organophosphates like trichlorfon can cause physiological damage in fish.
Purpose of the Study:
- To investigate the molecular mechanisms of trichlorfon toxicity in tambaqui liver.
- To identify specific pathways and genes affected by trichlorfon exposure.
- To understand the hepatic response to this widely used antiparasitic agent.
Main Methods:
- RNA-Sequencing (RNA-Seq) was employed to analyze gene expression.
- Tambaqui fish were exposed to a specific concentration (0.435 mg/L) of trichlorfon.
- Hepatic tissue was sampled for molecular analysis.
Main Results:
- Trichlorfon exposure activated metabolic pathways associated with tumor processes, immune responses, and apoptosis.
- Upregulation of solute carrier (SLC) genes was observed, potentially aiding trichlorfon uptake.
- Significant changes in gene expression indicate a complex cellular response to trichlorfon.
Conclusions:
- Trichlorfon significantly impacts tambaqui liver at the molecular level.
- The findings highlight potential risks of organophosphate use in aquaculture.
- Further research is needed to fully understand the long-term effects on fish health and ecosystems.
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