The distribution of actin in sheep ovaries

The distribution of actin in sheep ovaries has been examined using immunofluorescent techniques. The germinal epithelium, which contained abundant microfibrils, fluoresced strongly while the underlying stroma was only weakly positive. During early follicular growth a band of fluorescence was associated with the base of those granulosa cells abutting onto the basal lamina. This transitional polarity of actin distribution was correlated with cell shape changes in the basal granulosa.