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Updated: Sep 18, 2025
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
Comparative study of the glucose and trehalose addition on the extenders for goat sperm liquid storage
Md Emtiaj Alam1, Most Shorifa Yeasmin2, Dipak Kumar Das1
1Department of Veterinary and Animal Sciences, Faculty of Veterinary and Animal Sciences, Rajshahi University, Rajshahi, Bangladesh.
Objectives:
The objective of this study was to assess the effects of varying glucose and trehalose concentrations on tris-citric acid-egg yolk-fructose (TCEF) diluents for the short-term cold storage of goat semen.
Materials And Methods:
The semen sample was collected, unwashed, and divided into the following groups: control (TCEF without glucose and trehalose), TCEF + glucose (75, 150 mm), and TCEF + trehalose (75, 150 mm). Each experimental sperm group (sperm concentration: 9 × ×107/ml) was kept at 4°C in a refrigerator. The impact of varying glucose and trehalose levels on the quality of the spermatozoa was assessed at different time points: after dilution and at 5, 24, 48, and 72 h of refrigeration.
Results:
After dilution, progressive motility (PM), total motility (TM), sperm viability (SV), functional integrity (FI), and acrosome integrity of G-75, G-150, T-75, and T-150 did not differ significantly from the control. The PM, TM, SV, FI, and acrosome integrity of sperm of T-150 were considerably lower than the control, G-75, G-150, and T-75 after 5 and 24 h of cool storage. The T-75 group showed superior PM, TM, and FI after 48 h of cool storage, with noticeably greater values than the other groups.
Conclusion:
This study indicates that trehalose is a more favorable sugar than glucose for 48 h cool storage of buck semen, providing greater advantages in PM, TM, and PMI.
