Related Experiment Videos
Aminoglycoside peritoneal lavage: lack of efficacy in experimental fecal peritonitis
Abstract:
The utility of an adjunctive aminoglycoside lavage in the prevention of intra-abdominal abscesses utilizing an experimental rodent model was studied. Peritonitis was created in 115 rats by the intra-abdominal placement of gelatin capsules containing a barium-sulfate, human stool mixture. Four hours later, the animals were re-explored and lavaged with 30 ml/kg of sterile saline. Following the lavage, they were randomized to receive 15 ml/kg of either a 0.2% gentamicin or a normal saline lavage. The animals were then treated with intramuscular gentamicin-clindamycin or saline placebo for nine days. There was no difference in the number of abscesses between the groups receiving systemic antibiotics. The addition of gentamicin to a peritoneal lavage did not decrease the incidence of intra-abdominal infection in animals receiving effective systemic antibiotics.
Insights
Adjunctive aminoglycoside lavage did not prevent intra-abdominal abscesses in rats when combined with effective systemic antibiotics. This study found no significant difference in infection rates between groups, questioning its utility in preventing abscesses.
Area of Science:
- Surgical infection research
- Experimental surgery
- Pharmacology
Background:
- Intra-abdominal abscesses are a significant complication following abdominal surgery.
- Preventative strategies are crucial to reduce patient morbidity and mortality.
- The role of adjunctive peritoneal lavage with antibiotics requires further investigation.
Purpose of the Study:
- To evaluate the efficacy of adjunctive aminoglycoside lavage in preventing intra-abdominal abscesses.
- To determine if gentamicin lavage reduces infection incidence when used with systemic antibiotics.
Main Methods:
- A rodent model of peritonitis was established using gelatin capsules with stool mixture.
- Animals underwent initial lavage with sterile saline, followed by randomization to gentamicin or saline lavage.
- Post-lavage treatment involved intramuscular gentamicin-clindamycin or placebo for nine days.
Main Results:
- No significant difference in the number of intra-abdominal abscesses was observed between groups receiving systemic antibiotics.
- The addition of gentamicin to peritoneal lavage did not decrease infection incidence in animals on effective systemic therapy.
Conclusions:
- Adjunctive aminoglycoside lavage does not offer additional benefit in preventing intra-abdominal infections when effective systemic antibiotics are administered.
- Current findings suggest that gentamicin lavage is not a useful adjunct for preventing abscesses in this context.