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Functional Outlook of Penicillium digitatum PdMFS6 Transporter to Elucidate Its Role in Fungicide Resistance and

Paloma Sánchez-Torres1

  • 1Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Catedrático Agustín Escardino Benlloch 7, 46980 Paterna, Valencia, Spain.

Microorganisms
|June 27, 2025
PubMed

Insights

A novel transporter, PdMFS6, in Penicillium digitatum confers resistance to fungicides and enhances fungal virulence during citrus fruit infection. Deleting the gene reduces virulence, while overexpression increases chemical resistance and disease severity.

Area of Science:

  • Plant Pathology
  • Mycology
  • Molecular Biology

Background:

  • Penicillium digitatum causes postharvest rot in citrus fruits.
  • Fungicide resistance and virulence are critical factors in managing plant diseases.

Purpose of the Study:

  • To investigate the role of a novel MFS transporter, PdMFS6, in Penicillium digitatum.
  • To determine PdMFS6's involvement in fungicide resistance and fungal virulence.

Main Methods:

  • Gene deletion and overexpression of PdMFS6 in Penicillium digitatum.
  • Assessing chemical susceptibility and fungal virulence on citrus fruits.
  • Analyzing PdMFS6 gene transcription levels.

Main Results:

  • PdMFS6 deletion mutants showed no change in fungicide susceptibility.
  • Overexpression of PdMFS6 led to severe impairment in chemical susceptibility and increased resistance.
  • PdMFS6 deletion reduced fungal virulence on citrus fruit, while overexpression in specific strains enhanced disease signs.
  • PdMFS6 gene expression increased over time and was augmented in wild-type strains during fruit infection, indicating a role in infectivity.

Conclusions:

  • The fungal transporter PdMFS6 contributes to fungicide susceptibility in postharvest treatments.
  • PdMFS6 plays a significant role in enhancing the virulence of Penicillium digitatum during citrus fruit infection.
Keywords:
MDRMFS transporterPenicillium digitatumcitrusfungicide susceptibilitypostharvestvirulence

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