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Post-Exercise Whey Protein Supplementation: Effects on IGF-1, Strength, and Body Composition in Pre-Menopausal Women,
Marc Murray1, Lara Vlietstra1, Alyssa M D Best1
1School of Physical Education Sport & Exercise Sciences, University of Otago, Dunedin 9054, New Zealand.
Nutrients
|June 27, 2025
Summary
This study found that while combined training improved strength and lean mass in untrained women, post-exercise protein supplementation showed minimal impact on these outcomes or insulin-like growth factor-1 (IGF-1) levels.
Area of Science:
- Exercise Physiology
- Sports Nutrition
- Human Performance
Background:
- Untrained pre-menopausal women often seek effective training and nutritional strategies to enhance body composition and strength.
- The role of post-exercise protein supplementation in conjunction with combined training modalities requires further investigation.
Purpose of the Study:
- To assess the impact of post-exercise protein supplementation on insulin-like growth factor-1 (IGF-1), strength, and body composition.
- To evaluate the effects of a 12-week combined resistance and interval training program with varying protein intake.
Main Methods:
- Twenty-seven untrained pre-menopausal women were randomized into control or moderate protein groups.
- Participants completed 12 weeks of combined resistance and high-intensity interval cycle training.
- Measurements included IGF-1 concentration, 3-repetition maximum (3RM) strength, and dual-energy X-ray absorptiometry (DXA) for body composition.
Main Results:
- Both groups showed significant increases in strength (19-113%) and total lean mass (p=0.009).
- Post-exercise protein supplementation did not significantly alter IGF-1 levels or enhance strength and lean mass gains compared to the control group.
- The protein group had a significantly higher daily protein intake (~20 g/day).
Conclusions:
- Combined resistance and interval training effectively improves strength and lean mass in untrained women.
- Post-exercise protein supplementation appears to have a limited effect on these adaptations when added to a combined training program.
- IGF-1 was not found to be associated with strength or body composition changes in this cohort.

